Membrane-dependent reactions and blood coagulation

Blood coagulation is a large reaction network (in the essence, a multistep proteolytic cascade with feedbacks) in plasma that functions to locally convert blood from fluid state into a gelatinous one, and thus to stop bleeding. In addition to this hemostatic role, coagulation has also additional vital functions in immunity and angiogenesis. The two hemostatic mechanisms, platelet aggregation and blood coagulation, work side-by-side and complement each other.

In particular, all main reactions of blood plasma coagulation cascade do not occur in plasma. They proceed on the membranes of activated platelets, endotelial cells, microparticles and other surfaces available in blood. Molecular mechanisms of these two-dimensional surface-dependent reactions are very much biophysical in their nature, and are very hard to decypher from the experimental data: too many components, two-many unstable intermediates, and the general complexity of dealing with membranes on top of that. Investigation of the membran-dependend process using experiments and modelling, and elucidation of the overall regulation of the clotting network are among our priorities and the main areas of expertise.

gallery/bloodcoagulationdecomposed-1158

Substrate delivery mechanism and the role of membrane curvature in factor X activation by extrinsic tenase.

Kovalenko TA, Panteleev MA, Sveshnikova AN.

J Theor Biol. 2017 Dec 21;435:125-133.

Co-ordinated spatial propagation of blood plasma clotting and fibrinolytic fronts.

Zhalyalov AS, Panteleev MA, Gracheva MA, Ataullakhanov FI, Shibeko AM.

PLoS One. 2017 Jul 7;12(7):e0180668.

Blood coagulation factors bound to procoagulant platelets are concentrated in their cap structures to promote clotting

Nadezhda A. Podoplelova, Anastasia N. Sveshnikova, Yana N. Kotova, Anita Eckly, Nicolas Receveur, Dmitry Yu. Nechipurenko, Sergey I. Obydennyi, Igor I. Kireev, Christian Gachet, Fazly I. Ataullakhanov, Pierre H. Mangin, Mikhail A. Panteleev

Blood 2016 :blood-2016-02-696898; doi:10.1182/blood-2016-02-696898

Hysteresis-like binding of coagulation factors x/xa to procoagulant activated platelets and phospholipids results from multistep association and membrane-dependent multimerization

Nadezhda A. Podoplelova, Anastasia N. Sveshnikova, James H. Kurasawa, Andrey G. Sarafanov, Chambost Herve, Sergey A. Vasil'ev, Irina A. Demina, Fazly I. Ataullakhanov, Alessi Marie-Christine, and Mikhail A. Panteleev. 

Biochimica et Biophysica Acta - Biomembranes, 1858(6):1216–1227, 2016

Panteleev MA, Dashkevich NM, Ataullakhanov FI.

Thromb Res. 2015 Oct;136(4):699-711. doi: 10.1016/j.thromres.2015.07.025. Epub 2015 Jul 29. Review.

Shibeko AM, Panteleev MA.

Brief Bioinform. 2015 Jun 27. pii: bbv040

Zakharova NV, Artemenko EO, Podoplelova NA, Sveshnikova AN, Demina IA, Ataullakhanov FI, Panteleev MA.

PLoS One. 2015 Feb 17;10(2):e0116665. doi: 10.1371/journal.pone.0116665. eCollection 2015.

Terentyeva VA, Sveshnikova AN, Panteleev MA.

J Theor Biol. 2015 Oct 7;382:235-43. doi: 10.1016/j.jtbi.2015.07.001. Epub 2015 Jul 14

Lipets E, Vlasova O, Urnova E, Margolin O, Soloveva A, Ostapushchenko O, Andersen J, Ataullakhanov F, Panteleev M.

PLoS One. 2014 Jan 31;9(1):e87692. doi: 10.1371/journal.pone.0087692. eCollection 2014.

Ataullakhanov FI, Dashkevich NM, Negrier C, Panteleev MA.

Expert Rev Hematol. 2013 Apr;6(2):111-3. doi: 10.1586/ehm.13.12.

Abaeva AA, Canault M, Kotova YN, Obydennyy SI, Yakimenko AO, Podoplelova NA, Kolyadko VN, Chambost H, Mazurov AV, Ataullakhanov FI, Nurden

AT, Alessi MC, Panteleev MA.

J Biol Chem. 2013 Oct 11;288(41):29621-32. doi: 10.1074/jbc.M113.474163. Epub 2013 Aug 30.

Dashkevich NM, Ovanesov MV, Balandina AN, Karamzin SS, Shestakov PI, Soshitova NP, Tokarev AA, Panteleev MA, Ataullakhanov FI.

Biophys J. 2012 Nov 21;103(10):2233-40. doi: 10.1016/j.bpj.2012.10.011. Epub 2012 Nov 20.

Topalov NN, Yakimenko AO, Canault M, Artemenko EO, Zakharova NV, Abaeva AA, Loosveld M, Ataullakhanov FI, Nurden AT, Alessi MC, Panteleev MA.

Arterioscler Thromb Vasc Biol. 2012 Oct;32(10):2475-83. doi: 10.1161/ATVBAHA.112.253765. Epub 2012 Jul 26.

Balandina AN, Shibeko AM, Kireev DA, Novikova AA, Shmirev II, Panteleev MA, Ataullakhanov FI.

Biophys J. 2011 Oct 19;101(8):1816-24. doi: 10.1016/j.bpj.2011.08.034.

Panteleev MA, Balandina AN, Lipets EN, Ovanesov MV, Ataullakhanov FI.

Biophys J. 2010 May 19;98(9):1751-61. doi: 10.1016/j.bpj.2010.01.027.

Shibeko AM, Lobanova ES, Panteleev MA, Ataullakhanov FI.

BMC Syst Biol. 2010 Jan 26;4:5. doi: 10.1186/1752-0509-4-5.

Sinauridze EI, Kireev DA, Popenko NY, Pichugin AV, Panteleev MA, Krymskaya OV, Ataullakhanov FI.

Thromb Haemost. 2007 Mar;97(3):425-34.

Panteleev MA, Ananyeva NM, Greco NJ, Ataullakhanov FI, Saenko EL.

FEBS J. 2006 Jan;273(2):374-87.

Panteleev MA, Ovanesov MV, Kireev DA, Shibeko AM, Sinauridze EI, Ananyeva NM, Butylin AA, Saenko EL, Ataullakhanov FI.

Biophys J. 2006 Mar 1;90(5):1489-500. Epub 2005 Dec 2.

Panteleev MA, Ananyeva NM, Greco NJ, Ataullakhanov FI, Saenko EL.

J Thromb Haemost. 2005 Nov;3(11):2545-53.

Panteleev MA, Saenko EL, Ananyeva NM, Ataullakhanov FI.

Biochem J. 2004 Aug 1;381(Pt 3):779-94.

Panteleev MA, Zarnitsina VI, Ataullakhanov FI.

Eur J Biochem. 2002 Apr;269(8):2016-31.